FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n01_ogla_113.3510000008cd8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n01_ogla_113.3510000008cd8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29665809
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185400.017.7531575
GTATTGG272300.016.5233141
GTATAGG136850.015.8659541
GTATAAG135750.015.609531
TATACCG97650.015.467035
TATACAG177400.015.448075
GTCTAGG110000.015.2899271
CCTAGAC101150.015.0743
GTGTAGG132050.015.0034951
TACACTG138800.014.7824485
TACACCG97900.014.7483335
ACTGTGC132350.014.6770568
TAGACTG97450.014.5727455
CTATACT192700.014.5678394
CCTATAC134700.014.3168623
TACACAG135100.014.2029345
GTATATA397850.014.1870431
TAGACAG111750.014.1530085
TATTGGG306050.013.8446152
CCTACAC123250.013.8355653
Produced by FastQC (version 0.11.2)