FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n01_ogla_112.3510000008cdf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n01_ogla_112.3510000008cdf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23830559
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG152150.017.5746615
GTATAGG106450.016.9158381
GTATTGG199250.016.524751
TATACCG76550.015.9455125
TACACAG106000.015.0999175
TATACAG136750.015.0039965
CTATACT151300.014.9124484
CCTAGAC74900.014.776393
GTATAAG109300.014.6925281
CCTATAC106750.014.5503733
GTATATA313250.014.166281
CCTACAC99100.014.1397793
TACACCG80700.014.1249925
TACACTG108100.014.0157235
GTGTAGG102950.013.8450611
ACAGTGC114750.013.7825138
ACTGTGC108800.013.7505018
GTCTAGG87200.013.7304281
GTATAGA129850.013.7211121
TCTATAC122650.013.7097643
Produced by FastQC (version 0.11.2)