FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n01_ogla_111.3510000008ce7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n01_ogla_111.3510000008ce7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30846502
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG189650.016.6789175
GTATAGG134600.016.4830671
TATACCG99300.016.1185935
TACACTG130350.015.4125975
GTATATA407850.014.642021
CCTAGAC92450.014.5912013
ACTGTGC126650.014.5875548
CCTATAC133150.014.5188693
CTATACT196550.014.4271594
GTATTGG257250.014.3862711
TATACAG173000.014.3033625
TAGACTG98700.014.0511625
GTATAAG143000.014.0198251
TCTATAC161550.013.7894023
TTATACT233700.013.5564724
TAGACAG107850.013.5196285
TACACCG94500.013.4694395
TAGACGG51500.013.4645595
ACAGTGC140800.013.3576658
GTATAGA166900.013.093861
Produced by FastQC (version 0.11.2)