FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_122.3520000008cda2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_122.3520000008cda2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78740934
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG238600.016.6429375
TATACTG465150.016.4001055
GTATAAG343100.014.9104411
CTATACT471800.014.7695184
GTATTGG684450.014.6709681
GTATATA960750.014.4416461
TATACAG409450.014.408375
TTATACT566450.014.3560944
GTATAGG321250.014.0467531
TAATACT614750.013.9931034
CCTATAC321350.013.8797693
GTATTAT565250.013.8151861
GTATAAT540850.013.4899371
GTATTAA510100.013.4743781
GTATTAG378350.013.4208341
TAGACTG247750.013.0756975
ATAATAC558650.013.0175833
CGCCGTA373150.012.98557994-95
TCTATAC381700.012.9172523
ACTGTGC319000.012.8943298
Produced by FastQC (version 0.11.2)