FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_121.3520000008ce2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_121.3520000008ce2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51155991
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG292350.016.2151745
TATACCG144050.015.5970355
CGCCGTA275550.015.22325694-95
TTATACT367550.015.1979524
GTATAAG225100.015.0666861
GTATAGG201000.014.7463171
GTATATA614850.014.6088951
GTATTGG387600.014.5343621
GTATTAT373500.014.3708631
TACACTG201100.014.3610345
TATACAG260400.014.3375765
CTATACT292750.013.6780714
CCTATAC206350.013.4201913
GTATTAG248250.013.2215941
GTATAGA258250.013.0590941
TCTATAC246550.012.965963
ATTATAC351150.012.9182983
GTATTAA336050.012.7779021
TAATACT401900.012.7762054
GTATAAT350850.012.7668941