FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_119.3520000008cea1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_119.3520000008cea1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45529225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG198350.015.6853391
GTATATA520650.015.3723011
TATACCG116150.015.3354285
CGCCGTA252550.015.22697694-95
TATACAG208400.014.9972655
GTATAGG167900.014.852291
TATACTG249800.014.7174445
CTATACT247500.014.6814894
TGGTCGC583100.014.359200594-95
GTATTAT337650.014.2082131
GGTCGCC571750.013.94215994-95
GTATTAC177450.013.7852951
GTATTGG335600.013.7005581
CCTATAC168050.013.680163
TAATACT355150.013.5481974
TTATACT320200.013.51390654
GTATAGA224600.013.5137511
GTATTAG210100.013.4290481
TCTATAC215900.013.3102763
GTATAAT308000.013.2472961
Produced by FastQC (version 0.11.2)