FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_117.3520000008cfa0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_117.3520000008cfa0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25331709
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG149550.017.3738925
TATACCG76200.015.9580985
TTATACT185500.015.2103324
GTATATA314400.014.8665291
TATACAG136950.014.7755535
GTATTAT179200.014.5787981
GTATTGG225600.014.401711
GTATTAC105750.014.1041311
GTATAAG107850.014.093761
GTATAAT170250.014.0896861
GTATAGG102700.013.9217261
TACACCG77950.013.5279835
CTATACT151800.013.5178674
CCTATAC105750.013.430343
GTATTAG124750.013.3648351
ATAATAC185800.013.3450743
ATTATAC177000.013.28397753
GTATTAA167300.013.2308281
CGCCGTA117150.013.158874594-95
GTATAGA128750.013.1340851
Produced by FastQC (version 0.11.2)