FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_116.3520000008d018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_116.3520000008d018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29367821
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG181000.015.9039955
CGCCGTA158100.015.11416694-95
GTATAAG134750.015.0525911
TATACAG161850.014.7921065
GTATAGG121700.014.7541231
CTATACT182500.014.3937654
GTATATA382600.014.3524181
TACACTG123650.014.1372815
TATACCG88250.013.9949325
GTATTAT219500.013.9368151
GTATTGG256250.013.9030341
TAATACT230350.013.8577664
GTATAAT203550.013.6286841
ATAATAC210000.013.07436853
CCTATAC122600.013.0572463
TTATACT218350.013.0094874
GTATTAG143750.012.9866421
GTACTAG204000.012.8767771
TCTATAC144900.012.8507923
GTATTAC122750.012.7317061
Produced by FastQC (version 0.11.2)