FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n02_ogla_115.3520000008d082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n02_ogla_115.3520000008d082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642461
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA64950.016.78576594-95
TATACCG39450.016.6159445
GTATTGG120600.015.6364391
GTATAAG56300.015.4396471
TATACTG83800.015.3042735
GTATAGG57700.015.0650281
GTATTAT97850.014.5145981
GTATATA169950.014.4219251
GTATTAA88500.014.3305431
CTATACT83800.013.8872114
GCCGTAT55000.013.56046494-95
GTATAAT93450.013.4698021
TAGACTG42350.013.4592485
TTATACT100250.013.40895754
TATTGGG138200.013.1295552
GGTGCGT89400.013.1235817
TACACTG58650.013.0391965
TATACAG72650.012.9456095
ATAATAC94450.012.8745173
ACTGTGC54850.012.8160448