FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n01_ogla_122.3510000008cda5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n01_ogla_122.3510000008cda5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78740934
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG468750.017.275515
TATACCG237900.016.3706975
GTATAGG317850.015.0357471
CTATACT471600.014.9255144
TATACAG409900.014.8660815
TTATACT576350.014.783974
TAATACT608050.014.7318524
GTATATA964150.014.6290071
GTATAAG339300.014.6172581
GTATAGA404650.014.2524131
GTATTGG686350.014.2030291
TACACTG330850.014.0396255
GTATAAT535950.013.9340541
CCTATAC324700.013.7212753
GTATTAT562900.013.68891051
ATAATAC568700.013.5720073
ACTGTGC321750.013.5509748
TCTATAC384250.013.5231353
ATTATAC540950.013.2145793
TACACAG311150.013.1578585