FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n01_ogla_121.3510000008ce2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n01_ogla_121.3510000008ce2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51155991
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG301500.017.832235
TATACCG149050.016.5060945
GTATAAG220000.015.4394641
GTATTAT382150.014.8677821
CTATACT297950.014.8248524
TAATACT406750.014.5492734
ATAATAC369500.014.46134953
TTATACT367150.014.3074544
TATACAG260900.014.2539175
GTATATA624700.014.0913561
GTATAGG206000.014.0673241
TACACTG212500.014.0361255
TCTATAC246400.013.9176913
GTATTAG247700.013.6937071
GTATAGA250700.013.64353751
GTATAAT355600.013.6399531
ATTATAC347500.013.4496533
GTATTGG392950.013.4073551
TAGACTG154800.013.2850595
TACACAG195600.013.2092025