FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n01_ogla_119.3510000008cea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n01_ogla_119.3510000008cea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45529225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG248900.016.0482395
TATACCG117500.016.0071335
CTATACT247950.015.7652234
GTATATA521600.015.06511
TACACTG163300.014.7461315
GTATAAG194500.014.6312461
TAGACTG129600.014.4026975
TATACAG210250.014.3673735
GTATTGG322400.014.2054861
TTATACT325350.014.131524
CCTATAC171250.014.1179333
GTATAGA216600.014.0596231
TCTATAC209850.014.0561563
GTATAGG171650.014.0049261
TAATACT352600.013.9957974
GTATAAT308150.013.9681891
TACACCG120350.013.9310835
GTATTAT331050.013.819961
ATAATAC333000.013.7504443
GTGTAGG154800.013.7186271