FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n01_ogla_117.3510000008cfa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n01_ogla_117.3510000008cfa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25331709
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG75150.017.5067625
TATACTG156100.016.8562855
TATACAG137100.015.3469225
GTATTGG226050.015.3211751
GTATTAT186850.015.0266931
GTATATA320600.014.655541
ACTGTGC105350.014.5168398
CTATACT156400.014.303654
TTATACT189450.014.2652854
TAATACT198350.014.128064
TACACTG115500.014.1048085
ATTATAC172250.013.9255943
GTATAAG108450.013.8428851
TCTATAC125800.013.8191743
GTATTAG121350.013.4675231
TAGACTG77000.013.3851765
CTACACT109750.013.3726264
GTATAGG106250.013.3693811
GTATAAT174700.013.3251541
GTGTAGG100000.013.2072951