FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l03n01_ogla_115.3510000008d085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l03n01_ogla_115.3510000008d085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642461
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG79700.016.3879135
GTATAGG55650.016.2196881
TATACCG42350.016.1494715
TATACAG76100.015.35322955
TACACTG56600.015.2722925
GTATTGG115700.014.781661
GTATAAG57150.014.7133341
CCTATAC58800.014.54019453
GTATAAT97150.014.3766791
TAGACTG41550.014.2885535
TTATACT102000.014.0624584
CTATACT84650.014.0270864
TACACCG37850.013.9285645
GTATATA177200.013.8873741
TAATACT107700.013.7592044
TAGACAG46050.013.7173835
ACTGTCC39100.013.6046948
GTATTAG64450.013.5627871
CTACACT60350.013.5364754
GTATTAT100500.013.3774841