FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_76.3520000008d1d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_76.3520000008d1d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16558542
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC881070.5320939488512938No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG715390.4320368302958074No Hit
ACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAA366150.2211245410374899No Hit
GTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGG336380.20314590499574178No Hit
GGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAG166820.10074558496756539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG129600.021.845455
CTATACT138100.018.5746144
TTATACT189850.018.2655164
TACACTG85850.018.259695
CTACACT87200.017.8680464
TAATACT195200.017.3998684
TATACAG108350.017.1860835
TAGACTG54350.017.1307455
ACTGTGC72200.016.710328
GTATAGG78200.016.7055721
GTATTAG104300.016.669871
TAGGCTG63550.016.668985
GTATAAT165050.016.6647381
GTATTGG152350.016.5884041
TCTATAC112050.016.5765363
ATACTGT129600.016.4940476
TAGACAG58450.016.4167275
CTGTGCA97200.016.1252239
TATACCG54300.016.0092495
TAAGACT81350.015.766164
Produced by FastQC (version 0.11.2)