FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_62.3520000008d0c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_62.3520000008d0c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21675973
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG157100.021.8303835
CTATACT190500.019.1000354
TTATACT243950.018.8289454
TAATACT247800.018.3830534
CCTATAC127350.017.680023
ACTGTGC97850.017.4751138
TAGACAG72850.017.213725
GTATAGG124600.017.0410291
ATACTGT164100.016.7598196
TAGGACT92150.016.4951024
CTACACT106700.016.4271534
TAGACTG70350.016.204945
GTATTAC120250.016.1959021
TATACCG70500.016.1704645
CTGTGCG44250.015.8856039
GTATTAG138750.015.816681
GTATTAT217150.015.7499451
TATACAG140900.015.6762535
TCTATAC146600.015.42326453
TACACTG106350.015.4092655
Produced by FastQC (version 0.11.2)