FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_61.3520000008ce44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_61.3520000008ce44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291896
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67000.023.7504235
GTATAGG58750.022.6388231
TAGACTG31600.022.3975495
CTATACT91350.021.5794754
TACACTG49700.020.835385
TAATACT96150.020.057564
CTACACT47600.019.2597964
ATACTGT64800.018.8389686
CCTATAC67200.018.6611463
TAGACAG31600.018.4892525
ACTGTGC47800.018.3833278
CTAGACT37000.018.3584354
TATACAG57500.018.0916255
ACAGTGC53500.018.0228128
TATAGGG58500.017.7012632
GTATTGG70600.017.5606041
TTATACT100150.017.359274
ACTGTCC31950.017.0965028
TCTATAC58700.016.9935783
GACTGTG28300.016.9525097
Produced by FastQC (version 0.11.2)