FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_59.3520000008cf43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_59.3520000008cf43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19652835
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG141350.020.5331525
CTATACT165150.019.0409954
TTATACT214600.017.929394
TAATACT228950.017.905254
ACTGTGC95000.017.7991878
GTATAGG114500.017.4661351
ATACTGT137700.017.24836
TAGACTG65100.017.2203225
GTGTAGG76050.017.0523261
GTATTGG139450.016.3850121
CTGTGCG42500.016.2040719
TACACTG100300.016.1023125
GTATTAG120950.015.8277571
TCTATAC128550.015.4831313
GTATTAT191000.015.4695331
CCTATAC120000.015.4383273
AATACTG115950.015.4037815
ACTGTGT112050.015.3875178
ATAATAC185100.015.3722453
GTATAGA138300.015.3190861
Produced by FastQC (version 0.11.2)