FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_41.3520000008d14e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_41.3520000008d14e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17469344
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC243910.1396217282114314No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG205680.11773767807194133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133350.021.8711595
TAATACT203500.018.7200244
TTATACT196000.018.5638964
ATACTGT137100.018.3626296
CTATACT140950.018.1980614
ACTGTGC84150.017.2720498
GTATAAG99550.017.0822241
GTATAGG88500.017.0144791
TAGACTG59900.016.9700585
GTATTAT178550.016.8401721
GTATAAT177800.016.4303191
ATAATAC169250.016.249833
TATACCG61200.016.221515
TCTATAC117500.016.2108363
TATACAG123200.016.1932875
GTATTAG110600.016.0197731
AATACTG116250.015.8538685
TAGTACT213400.015.6702064
GTATAGA123200.015.5766231
CTGTGCG39400.015.5503059
Produced by FastQC (version 0.11.2)