FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_4.3520000008d2d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_4.3520000008d2d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10260648
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG75650.022.1658545
TTATACT102150.019.2522134
TAATACT106350.018.8938964
CTATACT75500.018.7494264
GTATTAT96550.018.7456231
GTATAAT95950.017.724141
TATACAG71400.017.564045
ATTATAC92800.017.5577583
TATACCG33300.017.5460575
ATACTGT77450.017.4800286
TACACTG53050.017.1923225
GTATAAG55650.017.157661
ATAATAC89900.017.0144963
ACTGTGC48100.016.3927578
GTATAGG44850.015.8875151
CCTATAC48650.015.7204863
ACAGTGC51900.015.5586038
GTATTGG82300.015.4689951
GTATATA176050.015.4343081
GTATAGA66250.015.2728771
Produced by FastQC (version 0.11.2)