FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_182.3520000008d04f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_182.3520000008d04f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62101033
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG374600.022.140331
CTATACT518850.020.4887774
CCTATAC417350.020.1682113
TATACTG387400.018.1467915
TATAGGG378250.017.393132
CCCTATA400200.016.9495432
TATACTA720650.016.4453815
TATACAG366800.015.8378035
GTATTAT470250.015.6469681
TAATACT509400.015.432524
TATACCG196800.015.1818035
GTATATA891650.015.0604851
TTATACT513450.014.9684954
TACACTG290650.014.5451255
TAGACTG199200.014.1642925
ATAATAC449700.014.1330973
ACTGTGC279950.014.0653048
GTATTGG401850.013.9366181
TACTAAC457200.013.8283267
TCTATAC315550.013.6384213
Produced by FastQC (version 0.11.2)