FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_164.3520000008ceca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_164.3520000008ceca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27192019
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG166750.022.5331821
CTATACT224900.021.754754
CCTATAC175650.021.0668133
TATACTG180600.020.5681535
TATACCG87250.018.0750275
CCCTATA163900.017.969032
TAATACT250700.017.6969684
TTATACT246400.017.5816824
TATAGGG165300.017.5007362
TATACAG161650.017.0728765
TATACTA325400.016.6123475
GTATTAT218800.016.608331
ATAATAC211800.016.5964533
GTATAAT223950.016.162771
ATTATAC214500.016.1218013
ATACTGT192400.015.7020936
GTATTAC137650.015.70171451
GTATATA410800.015.5410631
GTATTGG191000.015.3946271
GTATAGA155650.015.32027151
Produced by FastQC (version 0.11.2)