FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_137.3520000008d25a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_137.3520000008d25a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12079105
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG89750.022.8629765
GTATAGG75300.022.4565931
CTATACT115100.022.0368464
CTGTGCG26100.019.2890599
TAATACT141000.018.6963734
TAGACTG40650.018.695755
TTATACT140100.018.646964
ATACTGT91100.018.613656
ACTGTGC58400.018.4621438
CCTATAC82100.018.22433
TACACTG61000.017.052885
TAGACAG38650.016.7137095
TCTATAC81450.016.6202373
GTATTAT121550.016.4909761
TATAGGG73200.016.1575432
AATACTG73200.016.027635
TAGGACT53200.015.8924864
GTATAGA87000.015.8877561
ATAATAC117800.015.8867253
GTATAAG71800.015.8772631
Produced by FastQC (version 0.11.2)