FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_111.3520000008cfc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_111.3520000008cfc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27628206
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG182150.020.4193385
GTATAGG146200.019.6248551
CTATACT202200.018.770454
TAATACT235800.018.2109974
GTATTAT218650.017.923441
ATAATAC210050.017.074143
TTATACT245250.017.0638084
GTATAAG138750.016.6729681
TATACAG173050.016.4149155
GTATATA409500.016.3793891
CCTATAC145800.015.9970013
ATTATAC214950.015.9777413
TATACCG100100.015.6598995
GTATAGA156200.015.6010291
ACTGTGC131250.015.5976068
GTATTAG140000.015.5740511
TCTATAC143850.015.4873673
ACTATAC158200.015.1034483
TAGACTG94650.015.00581555
TATACTA290700.014.9841945
Produced by FastQC (version 0.11.2)