FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n02_osat_104.3520000008cdcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n02_osat_104.3520000008cdcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24888989
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG186650.021.7086185
CTATACT192850.019.0155454
TAGACTG89150.018.9687965
TTATACT259750.018.9276164
TACACTG132350.018.7351725
TAATACT262350.018.4322284
ACTGTGC128650.018.3129238
GTATAGG121050.017.698161
TAGACAG88600.017.3709035
TATACAG168800.017.0534135
ATACTGT191550.016.8879176
GTATTGG196050.016.8639281
CTACACT131850.016.6806134
TCTATAC157900.016.63633
GTATTAG147950.016.5993821
GTATAGA169450.016.4555661
TGTACTG189700.016.0509915
GTATTAT227250.015.9700351
TTACACT151100.015.8758894
GTATAAG134900.015.5641961
Produced by FastQC (version 0.11.2)