FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_76.3510000008d1d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_76.3510000008d1d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16558542
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC865060.5224252231869206No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG761560.45991971998500836No Hit
ACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAA362390.21885380971343976No Hit
GTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGG351460.2122529870081557No Hit
GGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAG175410.10593323977437144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125300.022.0240735
TTATACT186550.019.6563934
ACTGTGC74300.018.7291728
TAGACTG56050.018.6431665
CTATACT130700.018.2435744
TAATACT196650.017.4874464
TATACCG53650.017.3523755
TACACTG80000.017.2179165
ATACTGT126000.017.1129596
TATACAG103300.016.7368475
ACTGCGC28000.016.622948
TAAGACT79900.016.4075644
ATTATAC153050.016.1380773
AGTACTG100500.015.974355
ACAGTGC79850.015.9404178
GTATAGG79700.015.9164021
TCTATAC112400.015.8892453
GTATTGG159650.015.7129221
GTATTAG107800.015.6900091
GTATAAG99300.015.6455361
Produced by FastQC (version 0.11.2)