FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_62.3510000008d0c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_62.3510000008d0c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21675973
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG165300.020.1719045
CTATACT189350.018.4128674
TTATACT244550.017.9082814
GTATAGG120200.017.5104031
ACTGTGC101350.017.3860028
TAATACT248450.017.3403934
TAGACTG77850.017.2667435
TATACCG72350.016.4785065
CTAGACT84900.015.7772784
TACACTG105800.015.7132135
TATACAG146600.015.3253285
GTATTAT217100.015.3191631
CCTATAC125950.015.1983883
ATACTGT171000.015.19290356
TCTATAC146900.014.9709653
CTACACT105250.014.938144
ATTATAC209350.014.8613743
GTATAGA153700.014.8376191
ATAATAC210550.014.8217933
GTATTGG153350.014.7475561
Produced by FastQC (version 0.11.2)