FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_61.3510000008ce47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_61.3510000008ce47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291896
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG63350.024.7419955
GTATAGG63400.021.8057791
TTATACT95050.021.1875674
ATACTGT64550.020.9694926
CTATACT93550.020.9180324
TACACTG49250.020.5419045
CTGTGCG22400.019.718529
CTACACT48750.019.6808624
ACTGTGC47400.019.0377048
ACTGTGT54700.018.754518
TTACACT57400.018.5354654
CCTATAC68100.018.2734933
TAAGGCT40150.018.0997774
TAGACTG32700.017.575395
TAGACAG31000.017.4666865
ACAGTGC50250.017.3908628
TAACACT52750.017.3780984
CTGTACT51650.017.2884034
TACACAG45700.017.2527875
ACTGACC28450.017.1946878
Produced by FastQC (version 0.11.2)