FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_59.3510000008cf46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_59.3510000008cf46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19652835
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG136300.021.048125
TAGACTG64250.020.9950755
CTATACT163300.020.3025364
CTGTGCG42050.019.5402059
TTATACT215600.018.6161144
ACTGTGC89900.018.2267158
TAATACT228550.018.0185154
ATACTGT141800.017.1491576
ACTGCGC35550.017.1009168
TACACTG99350.017.0676065
GTATAGG118450.016.8066251
CCTATAC118950.016.6914063
ACTGTGT114700.016.3976218
TATACAG129450.016.3645865
AATACTG122250.015.851985
GTATTAT191100.015.7875441
TATCCTG98950.015.6485485
GTATAGA134700.015.6256351
TACTGTG102050.015.5447057
CTGTGCA144150.015.4528029
Produced by FastQC (version 0.11.2)