FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_41.3510000008d141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_41.3510000008d141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17469344
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC251950.1442240761874058No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG204980.11733697613373462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133500.021.8812895
TAATACT197950.019.1726254
CTATACT141300.018.9931324
TTATACT193550.018.724994
ACTGTGC81300.017.876358
ATACTGT132850.017.589426
TAGACTG65850.017.5278875
TATACAG122100.017.5055565
GTATTAT178850.016.7632711
TATACCG62450.016.6567695
TACACTG90800.016.5302855
GTATTAG112350.016.4933871
ATAATAC168050.016.422073
GTATAGA129750.016.3322411
TAAGACT88800.016.3146274
CCTATAC87900.015.887253
ATTATAC167550.015.8757353
ACTGCGC31450.015.8568398
GTATAAG101250.015.7205631
GTATAAT174500.015.7108141
Produced by FastQC (version 0.11.2)