FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_4.3510000008d2d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_4.3510000008d2d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10260648
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG74250.022.5176735
TAATACT103650.019.0178034
TTATACT102800.018.4819774
ATAATAC91350.017.314793
ATACTGT75700.017.2537066
CTATACT80300.016.917344
GTATTAT94950.016.6131381
ACTGTGC48150.016.4727368
GTATAAT93000.016.450591
TATACCG33650.016.3738295
GTATTAG60250.016.0872461
TATACAG74900.016.0441255
CTGTGCG25650.015.7399
TACACTG47800.015.6008795
ACAGTGC50600.015.5812818
ATTATAC91400.015.5384093
GTATTAA91200.015.4728461
TCTATAC63600.015.2354273
GTATAGA66150.015.0833571
ACTATAC64400.014.9724113
Produced by FastQC (version 0.11.2)