FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_182.3510000008d042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_182.3510000008d042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62101033
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG382250.021.093451
CTATACT519100.020.5702024
CCTATAC413400.020.234023
TATACTG383950.018.7423295
TATAGGG382100.017.1809442
CCCTATA397700.017.140062
TATACTA716000.016.233295
TATACCG197150.015.7808415
TAGACTG214650.015.755595
TAATACT521250.015.4642574
TATACAG374750.015.3746345
TAGACAG202400.014.8552115
TTATACT507300.014.6441854
ACTGTGC286050.014.4785768
TACACTG290850.014.4040675
GTATTAT464850.014.3402961
GTATATA886500.014.2512461
ATAATAC457550.014.1082843
GTATTGG399050.014.014011
TCTATAC327250.013.9197753
Produced by FastQC (version 0.11.2)