FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_164.3510000008cecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_164.3510000008cecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27192019
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT236800.021.8235914
GTATAGG163500.021.2416421
TATACTG183700.020.9951745
CCTATAC176050.019.8573283
TAATACT250050.018.2547364
TTATACT249300.017.795234
TATACAG164400.017.4216045
TATACCG89050.017.01495
GTATTAT217950.016.894031
ATAATAC214800.016.6509763
TATACTA333400.016.5258965
CCCTATA171750.016.372992
TCTATAC153900.016.0488433
TATAGGG162750.016.0233882
TAGACTG89250.015.8591795
GTATATA419650.015.7707491
GTATAGA153900.015.6206951
ACTGTGC126800.015.6192978
ATTATAC218500.015.6081713
GTATAAG136100.015.3597091
Produced by FastQC (version 0.11.2)