FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_137.3510000008d25d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_137.3510000008d25d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12079105
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG89500.023.5105045
GTATAGG81050.021.2208441
CTATACT113400.020.0636944
TAGACTG41850.019.408055
TACACTG58650.019.35585
TTATACT138050.019.164934
ATACTGT92150.019.0704366
ACTGTGC61800.019.0597638
TAATACT138100.018.6420674
CTGTGCG29950.017.6027479
CCTATAC78500.017.4870533
ATTATAC120650.016.9683023
TATACAG81850.016.5969625
TATAGGG76250.016.5713942
GTATTAG74800.016.515011
CTACACT60000.016.466474
AATACTG76250.016.3831425
GGACCGT23900.016.2955956
GTATTAT121350.016.2877351
GTATTAC68700.016.2524281
Produced by FastQC (version 0.11.2)