FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_111.3510000008cfcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_111.3510000008cfcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27628206
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG182200.020.8033925
CTATACT199900.018.5815324
TTATACT246450.018.5795734
TAATACT242450.018.2004074
GTATAGG145700.017.837051
ATAATAC216300.017.4581473
GTATTAT215300.017.3891121
TATACCG96550.016.9725275
ATTATAC219100.016.8231333
TATACAG179050.016.7126735
TAGACTG98350.015.9374655
CCTATAC148200.015.8972133
ATACTGT190100.015.6651196
GTATAAG137250.015.4043331
TACACTG135550.015.2780055
GTATTAC129450.015.0846411
GTATATA407550.015.0501421
TATACTA291500.014.9909425
GTATAAT223000.014.9138041
TTACACT147300.014.8334654
Produced by FastQC (version 0.11.2)