FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l02n01_osat_104.3510000008cdce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l02n01_osat_104.3510000008cdce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24888989
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG191050.021.4804175
TAGACTG89450.019.5408525
ACTGTGC119150.019.0934098
TACACTG130350.018.6931445
TTATACT257500.018.1325664
TAATACT265650.018.1126774
CTACACT125900.017.9959854
CTATACT193450.017.5557884
TATACAG167100.017.1970885
TAGACAG89700.017.1564965
TATACCG86950.017.1528425
ATACTGT196650.016.5439286
CTGTGCG63050.016.496859
GTATAAG137400.016.0450731
ATAATAC222300.015.9397813
CCTATAC117050.015.8667633
GTATTAT225150.015.827041
GACTGTG76600.015.8107837
TTACACT150150.015.65895754
TCTATAC155600.015.6294373
Produced by FastQC (version 0.11.2)