FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_84.3520000008cd1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_84.3520000008cd1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53924047
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG248700.017.6310251
CTATACT337050.016.9273094
TATACTG323800.016.7103395
CCTATAC262750.016.5435873
GTATATA628900.015.0699911
TATACAG297950.014.9713385
GTATTAT369550.014.5135091
GTATTAG237400.014.3079371
TATACCG142800.014.1383635
TAGACTG178200.014.1021835
GTATAGA261450.014.0456731
TAATACT378600.013.727014
TTATACT388100.013.6970064
ATAATAC368800.013.667083
TACACTG245500.013.6225865
GTATAAG227350.013.5195111
GTATAAT353650.013.44658851
TATACTA460300.013.3443045
TAGACAG171500.013.2681325
GTATTAA315200.013.2330521
Produced by FastQC (version 0.11.2)