FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_204.3520000008cfd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_204.3520000008cfd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34978160
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG207500.020.582161
CTATACT263950.019.3113174
TATACTG209250.018.207135
CCTATAC214500.018.027933
TATAGGG197350.017.307772
GTATATA439850.016.57881
GTATTAT246700.016.502931
TATACTA350650.015.958945
ATAATAC240300.015.8946513
TAATACT254350.015.8379134
TTATACT260400.015.8165574
GTATAAT243750.015.7866411
TATACAG208600.015.71335
CCCTATA204550.014.9799222
ATTATAC231900.014.8110663
TATACCG112100.014.6199575
GTATAGA177850.014.5843861
GTATAAG155950.014.5610221
TAATACA247750.014.5149674
TAGACTG113900.014.5140335