FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_183.3520000008cf50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_183.3520000008cf50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29958202
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG179900.018.1671035
CTATACT208200.017.5915554
GTATAGG139100.016.29061
GTATTAT218300.016.255961
CCTATAC145650.016.1777083
TATACAG173450.015.3370595
ATAATAC216500.015.09653
TTATACT232150.014.9377354
TAATACT236200.014.9229474
TATACCG96600.014.8510875
GTATATA402400.014.6625471
ATTATAC211700.014.2045873
GTATTAG139900.014.0242011
TAGACAG98800.013.8953445
GTATAAT223400.013.7796331
TATACTA293050.013.7137765
TACACTG132150.013.5879215
TCTATAC152250.013.5730583
GTATAAG135350.013.1970041
ATACTGT199350.013.1537556
Produced by FastQC (version 0.11.2)