FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_144.3520000008ce51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_144.3520000008ce51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58344761
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG352450.017.0632425
TAATACT469450.015.8565384
GTATATA774800.015.8433031
CTATACT355700.015.8390654
TTATACT454250.015.1531244
GTATTAT432900.015.0998881
GTATAGG252450.015.0353741
TATACAG344350.014.8711315
ATAATAC417300.014.4917933
TCTATAC285050.014.2657573
GTATTGG429000.014.0190721
ATTATAC398650.013.97810653
GTATAAT423050.013.8456771
GTATTAG283600.013.70218851
ACTGTGC259050.013.6968618
GTATAAG255500.013.6845261
TATACCG185200.013.6457055
TAGACTG196600.013.4826735
GTATAGA310200.013.4154471
TACACTG276550.013.2436015
Produced by FastQC (version 0.11.2)