FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_140.3520000008cdd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_140.3520000008cdd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18756721
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG126550.021.1713475
CTATACT145400.017.871234
TTATACT198250.017.5160314
ATACTGT131700.016.2675116
CTGTGCG44850.015.8852619
TAATACT203650.015.6519934
TACACTG87400.015.5991885
ACTGTGC83050.015.4425348
TAGACTG59350.015.287775
GTATTAT172500.015.2291491
TACTGTG96500.014.8168817
ACTGTGT100950.014.774678
GTATAGA120350.014.644261
GTATTGG136700.014.4912941
TTACACT111650.014.423564
TATACAG110000.014.2944295
CTACACT87250.014.2648554
CTGTGCA130300.014.2527249
TATACCG57650.014.0905625
TAAGACT87750.014.0753024
Produced by FastQC (version 0.11.2)