FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n02_osat_119.3520000008cd52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n02_osat_119.3520000008cd52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32650088
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG212650.018.7648075
TAATACT264800.018.2267234
CTATACT207250.017.2826234
GTATTAT250700.017.205781
TATACAG198050.016.8140895
GTATATA459600.016.517321
TTATACT267400.016.4506264
ATTATAC236150.016.3748473
GTATAGG149650.016.2848381
ATAATAC243450.016.0399463
GTATAAG152400.015.6480991
GTATTAG158100.015.3543651
GTATAGA178900.015.2951941
GTATTGG272150.015.2038161
TATACCG102500.014.9695545
TCTATAC164150.014.7883883
CCTATAC148200.014.6169743
GTATTAC163950.014.5746941
GTATAAT246900.014.48827651
TACACTG151850.014.3591285
Produced by FastQC (version 0.11.2)