FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_84.3510000008cd11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_84.3510000008cd11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53924047
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT345800.018.1593844
GTATAGG247950.017.4378261
TATACTG322450.017.4267565
CCTATAC265300.017.3315093
TATACCG138950.015.9643995
TACACTG256950.015.3804465
GTATTAT373400.015.0021881
TTATACT391250.014.8236774
GTATATA628200.014.6351811
TATACAG293600.014.6091835
TATACTA474800.014.4561075
CCCTATA237500.014.1003332
TAATACT386350.013.90516954
ATTATAC342250.013.8788913
GTATTAG237300.013.7554271
ATAATAC358100.013.75538353
TAGACTG184250.013.7408615
GTATAAT355000.013.5312491
ACTGTGC259150.013.5256678
TCTATAC254350.013.5022093
Produced by FastQC (version 0.11.2)