FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_204.3510000008cfd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_204.3510000008cfd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34978160
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG210600.019.741511
CTATACT267100.019.313154
CCTATAC217000.018.693813
TATACTG206100.018.1611585
TATACTA355150.016.5177335
TTATACT264050.016.1362824
GTATTAT242950.016.0958391
GTATTAG156150.016.0056921
TAATACT265900.015.9704224
GTATATA454350.015.7912721
ATAATAC241600.015.4337843
TATACAG207600.015.3071415
TATAGGG198450.015.0559422
ATTATAC233550.014.9488283
CCCTATA205950.014.9458832
TAGACTG119050.014.7228645
TATACCG109300.014.6889885
GTATAAG151900.014.4202841
ATACTGT223850.013.8771556
TACACTG162950.013.8463265