FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_183.3510000008cf53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_183.3510000008cf53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29958202
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG188500.018.0168765
CTATACT202250.017.7079524
TATACCG103000.016.9244145
GTATTAT220850.016.1359981
GTATAGG141350.015.7319321
GTATATA408100.015.3804221
CCTATAC141300.015.3289043
TAATACT244500.015.1336664
TTATACT243900.015.1319464
TATACAG179250.014.4948255
TAGACTG100000.014.3447095
GTATTAG146050.014.3147281
ATTATAC221750.014.1373753
TATACTA291850.014.0129075
TCTATAC151450.013.8311333
ACTGTGC137100.013.6493188
GTATAGA159450.013.5289261
GTATAAT221400.013.3703391
TACACTG137150.013.3336725
ATAATAC220350.013.2787153
Produced by FastQC (version 0.11.2)