FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_144.3510000008ce54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_144.3510000008ce54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58344761
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG366150.017.941545
TAATACT467250.016.336674
CTATACT370600.016.021444
TTATACT470000.015.9479944
GTATATA785600.015.428841
GTATTAT423700.015.0942271
TATACAG339000.015.0207515
ATAATAC427300.014.9183333
GTATAAT421450.014.6900271
ATTATAC423950.014.4087723
TACACTG279150.014.2594565
GTATAAG258350.014.2533681
GTATTGG421650.014.1083581
CCTATAC249200.014.0672433
TCTATAC288400.013.999883
GTATTAG273400.013.8684721
GTATAGG254750.013.8579461
TAGACAG194850.013.8466435
ATACTGT404600.013.7236836
TAGACTG190300.013.6784975
Produced by FastQC (version 0.11.2)