FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_140.3510000008cddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_140.3510000008cddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18756721
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG123400.018.745855
CTATACT146600.017.0104834
TTATACT194800.016.7760934
TAATACT205300.016.6353244
ACTGTGC84450.015.8599788
TATACAG112400.015.5515215
GTATTGG135450.015.2236581
TACACTG88250.015.1783915
GTATAGG100600.015.0661061
ATACTGT131350.014.8624486
CTGTGCA133050.014.778789
CTACACT88650.014.6812544
TAGACTG58250.014.6780345
TAGACAG61450.014.3774675
TCTATAC115450.014.235643
TTACACT110700.014.1598174
TAAGACT88250.014.1557334
GTATTAT169550.014.1514821
TATACCG60800.014.1405515
CCTATAC103350.014.0179393