FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l01n01_osat_119.3510000008cd55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l01n01_osat_119.3510000008cd55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32650088
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG211300.019.3329325
TAATACT266350.017.4059014
CTATACT214300.017.2004174
TTATACT276300.017.1916894
GTATTAT253100.017.04631
ATAATAC244100.016.4240173
TATACAG203300.016.145055
ATTATAC244100.015.8596843
GTATATA462250.015.758021
GTATAAT244550.015.4467071
ACTGTGC147300.015.3806898
TATACCG106550.015.3357045
ATACTGT224350.015.1166516
TAGACTG110350.014.9367415
TATACTT281500.014.8660735
GTATAGA173250.014.6180831
GTATAGG150450.014.5594561
ACTATAC178750.014.5094613
CCTATAC149350.014.5032173
GTATAAG153700.014.4679971