FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_99.35200000073a46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_99.35200000073a46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93041624
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG683750.020.6015135
CTATACT736100.019.0981064
TTATACT921000.019.0476974
TAATACT935250.018.9811444
GTATAGG504700.018.193241
GTATTAT825650.017.7039011
GTATTAG518500.016.8377881
ACTGTGC450700.016.7584138
ATAATAC800200.016.7305373
GTATAAT844750.016.6787911
TAGACTG330650.016.5346165
TATACAG617900.016.52655
CCTATAC513200.016.5080913
TATACCG312600.016.5008225
GTATAAG501750.016.2152521
ATTATAC818550.016.1289633
TACACTG473700.015.8167635
ATACTGT728600.015.7140996
TAGACAG322500.015.3751525
TATACTT953450.015.2676465