FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_220.35200000073b95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_220.35200000073b95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35016453
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG230350.020.973591
CTATACT285800.020.6102774
CCTATAC245750.020.1387233
TATACTG210150.019.0704865
TATACTA400400.016.8718835
CCCTATA226750.016.5880932
TAATACT289100.016.313174
TTATACT286100.016.1352674
TATACCG114050.015.9232475
GTATTAT259300.015.697791
TATAGGG218350.015.571132
TATACAG200000.015.4744265
GTATATA527100.015.1830261
ATAATAC255750.015.1501433
TAGACTG120550.015.0646725
TCTATAC175750.014.986123
GTATAAT257750.014.7959541
ATTATAC254100.014.7807743
GTATTAG162900.014.56621551
TACACTG164750.014.5146355